Journal: International Journal of Molecular Sciences

Article Title: Mitochondrial Dysfunction and Induction of Apoptosis in Hepatocellular Carcinoma and Cholangiocarcinoma Cell Lines by Thymoquinone

doi: 10.3390/ijms232314669

Figure Lengend Snippet: MMP disruption in HepG2 cells following exposure to TQ for 24 h (scale bar: 50 μm). MMP was evaluated using JC-1 by image techniques in the cancer cells after being exposed to 0–100 μM TQ: 0 μM TQ ( a ) and DMSO ( b ), and 10 ( c ), 25 ( d ), 50 ( e ), 75 ( f ), and 100 μM ( g ) TQ. The best representative images of each group are shown.

Article Snippet: Changes in the mitochondrial membrane potential (MMP or ΔΨm) were detected using the fluorescent JC-1 (5, 5′, 6, 6′-tetrachloro-1,1′,3,3′-tetraethyl-benzimidazolyl-carbocyanine iodide) dye (Molecular Probes, Invitrogen, Germany), which fluoresces red/orange fluorescence in intact and healthy mitochondria and fluoresces green fluorescence in the cytoplasm in disrupted mitochondria.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Mitochondrial Dysfunction and Induction of Apoptosis in Hepatocellular Carcinoma and Cholangiocarcinoma Cell Lines by Thymoquinone

doi: 10.3390/ijms232314669

Figure Lengend Snippet: The upper panel shows the rationale of using JC-1 to determine the depolarization/disruption of the mitochondrial membrane, with the three-color histograms of red, green, and blue fluorescence representing 0 TQ, DMSO, and 50 μM TQ for HepG2 cells, and the mitochondrial depolarization for HepG2 using JC-1. A decrease in the red (~590 nm)/green (~529 nm) fluorescence intensity ratio after exposure to TQ is indicative of depolarization/disruption of the mitochondrial membrane. Three independent experiments were carried out for each concentration. The variation in the mitochondrial depolarization is significant ( p < 0.001, two-tailed test). Red means healthy mitochondria (HMΨ) while green means unhealthy mitochondria (UHMΨ) (RGB, red, green, and blue fluorescence channels; for each fluorescence channel evaluation, there is mean, the standard deviation, and the mode).

Article Snippet: Changes in the mitochondrial membrane potential (MMP or ΔΨm) were detected using the fluorescent JC-1 (5, 5′, 6, 6′-tetrachloro-1,1′,3,3′-tetraethyl-benzimidazolyl-carbocyanine iodide) dye (Molecular Probes, Invitrogen, Germany), which fluoresces red/orange fluorescence in intact and healthy mitochondria and fluoresces green fluorescence in the cytoplasm in disrupted mitochondria.

Techniques: Fluorescence, Concentration Assay, Two Tailed Test, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Mitochondrial Dysfunction and Induction of Apoptosis in Hepatocellular Carcinoma and Cholangiocarcinoma Cell Lines by Thymoquinone

doi: 10.3390/ijms232314669

Figure Lengend Snippet: MMP disruption in HuCCT1 cells following exposure to TQ for 24 h (scale bar: 25 μm) MMP was evaluated using JC-1 by image techniques in the cancer cells after being exposed to 0–100 μM TQ: 0 μM TQ ( a ) and DMSO ( b ), and 10 ( c ), 25 ( d ), 50 ( e ), 75 ( f ), and 100 μM ( g ) TQ. The best representative images of each group are shown.

Article Snippet: Changes in the mitochondrial membrane potential (MMP or ΔΨm) were detected using the fluorescent JC-1 (5, 5′, 6, 6′-tetrachloro-1,1′,3,3′-tetraethyl-benzimidazolyl-carbocyanine iodide) dye (Molecular Probes, Invitrogen, Germany), which fluoresces red/orange fluorescence in intact and healthy mitochondria and fluoresces green fluorescence in the cytoplasm in disrupted mitochondria.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Mitochondrial Dysfunction and Induction of Apoptosis in Hepatocellular Carcinoma and Cholangiocarcinoma Cell Lines by Thymoquinone

doi: 10.3390/ijms232314669

Figure Lengend Snippet: Mitochondrial depolarization using JC-1 for HuCCT1 using image analysis is demonstrated for DMSO and several concentrations of TQ. Three independent experiments were carried out for each concentration. A decrease in the red (~590 nm)/green (~529 nm) fluorescence intensity ratio after exposure to TQ is indicative of depolarization/disruption of the mitochondrial membrane. Three independent experiments were carried out for each concentration. Red means healthy mitochondria (HMΨ) while green means unhealthy mitochondria (UHMΨ). The variation in the mitochondrial depolarization is significant ( p < 0.001, two-tailed test).

Article Snippet: Changes in the mitochondrial membrane potential (MMP or ΔΨm) were detected using the fluorescent JC-1 (5, 5′, 6, 6′-tetrachloro-1,1′,3,3′-tetraethyl-benzimidazolyl-carbocyanine iodide) dye (Molecular Probes, Invitrogen, Germany), which fluoresces red/orange fluorescence in intact and healthy mitochondria and fluoresces green fluorescence in the cytoplasm in disrupted mitochondria.

Techniques: Concentration Assay, Fluorescence, Two Tailed Test

Journal: PLoS ONE

Article Title: Bone Marrow Mesenchymal Stem Cells Attenuate Mitochondria Damage Induced by Hypoxia in Mouse Trophoblasts

doi: 10.1371/journal.pone.0153729

Figure Lengend Snippet: A : The mitochondrial membrane potential (ΔΨm) was tested by using JC-1; B : Mitochondrial ultramicrostructrue were detected by TEM [Ba~Bd: the most representative image of mitochondrial morphology and cristae of trophoblast cells cultured alone or co-cultured in normoxia and hypoxia (black arrow); Ba’~Bd’: higher magnification.] C : ATP levels of the two groups in normoxia and hypoxia; * p <0.05 vs. the corresponding control group.

Article Snippet: Mitochondrial stability was determined by fluorescence microscopy after incubation with JC-1 (5,59,6,69-tetrachloro-1,19,3,39-tetraethylben zimidazolylcarbocyanine iodide; Molecular Probes, Eugene, OR).

Techniques: Cell Culture